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Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

9,379 Views

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10:59 min

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May 12, 2019

DOI:

10.3791/59478-v

DOI:
10.3791/59478-v

•

10:59 min
May 12, 2019

•

3 Views
, , , , ,
1Wellcome Trust Sanger Institute, 2Department of Medicine,University of Cambridge, 3University of Cardiff

Summary

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Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids offer exciting opportunities to model enteric diseases in vitro. We demonstrate the differentiation of hiPSCs into intestinal organoids (iHOs), the stimulation of these iHOs with cytokines, and the microinjection of Salmonella Typhimurium into the iHO lumen, enabling the study of an epithelial invasion by this pathogen.

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Human Induced Pluripotent Stem CellsIntestinal OrganoidsEpithelial Cell ProtectionSalmonellaPathogensMicro-injectionDifferentiationRPMI-B27 MediaCHIR99021Retinoic AcidBasement Membrane MatrixCollagenaseOrganoid Base Growth MediaCentrifugation24-well Plate

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Lees, E. A., Forbester, J. L., Forrest, S., Kane, L., Goulding, D., Dougan, G. Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens. J. Vis. Exp. (147), e59478, doi:10.3791/59478 (2019).

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