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Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

9,293 Views

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10:59 min

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May 12, 2019

DOI:

10.3791/59478-v

DOI:
10.3791/59478-v

•

10:59 min
May 12, 2019

•

3 Views
, , , , ,
1Wellcome Trust Sanger Institute, 2Department of Medicine,University of Cambridge, 3University of Cardiff

Tags

Human Induced Pluripotent Stem CellsIntestinal OrganoidsEpithelial Cell ProtectionSalmonellaPathogensMicro-injectionDifferentiationRPMI-B27 MediaCHIR99021Retinoic AcidBasement Membrane MatrixCollagenaseOrganoid Base Growth MediaCentrifugation24-well Plate

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Lees, E. A., Forbester, J. L., Forrest, S., Kane, L., Goulding, D., Dougan, G. Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens. J. Vis. Exp. (147), e59478, doi:10.3791/59478 (2019).

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