ジャーナル
/
免疫学と感染
/
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

DOI:
10.3791/59478-v

•

10:59 min

•

May 12, 2019

•

, , , , ,
1Wellcome Trust Sanger Institute, 2医学部,University of Cambridge, 3University of Cardiff

章

  • 00:04標題
  • 00:38Differentiation from iPSCs to the Hindgut
  • 01:10Embedding of the Hindgut in Basement Membrane Matrix
  • 03:13Maintenance, Passage, and Pre-stimulation of iHOs with rhIL-22
  • 05:28Microinjection of iHOs and Intracellular Invasion Assays
  • 09:19Results: Studying Epithelial Cell Protection Against Pathogens Using hiPSC-derived iHOs
  • 10:05Conclusion

概要

自動翻訳

自動翻訳

Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids offer exciting opportunities to model enteric diseases in vitro. We demonstrate the differentiation of hiPSCs into intestinal organoids (iHOs), the stimulation of these iHOs with cytokines, and the microinjection of Salmonella Typhimurium into the iHO lumen, enabling the study of an epithelial invasion by this pathogen.

タグ

Human Induced Pluripotent Stem CellsIntestinal OrganoidsEpithelial Cell ProtectionSalmonellaPathogensMicro-injectionDifferentiationRPMI-B27 MediaCHIR99021Retinoic AcidBasement Membrane MatrixCollagenaseOrganoid Base Growth MediaCentrifugation24-well Plate

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Read Article