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Real-Time In Vitro Monitoring van Odorant Receptor activering door een Odorant in de damp-fase
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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Chapters
- 00:04Title
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fysiologisch, worden odorant receptoren geactiveerd door odorant moleculen in de fase van de damp ingeademd. Echter, meest in vitro systemen gebruiken vloeibare fase odorant stimulatie. Hier presenteren we een methode waarmee in vitro bewaking in real-time van odorant receptor activering op odorant stimulatie in damp fase.
