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Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
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Neuroscience
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JoVE Journal Neuroscience
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

DOI:
10.3791/52652-v

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10:16 min

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July 13, 2015

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,
1UMR Centre des Sciences du Goŭt et de l’Alimentation, CNRS, INRA,Université de Bourgogne

Chapters

  • 00:05Title
  • 01:14Pipette and Solution Preparations
  • 03:45Preparing the Olfactory Epithelium
  • 06:58Making Recordings
  • 08:47Results: Stimulating the MOR23 Receptor of Olfactory Receptor Neurons (ORNs) with Lyral
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.

Tags

Keywords: Olfactory Sensory NeuronsPatch-clamp RecordingOdorant ReceptorGenetically Tagged MiceGFPOlfactory EpitheliumMembrane PropertiesLigand-receptor InteractionsTransduction PathwaysPharmacologyCoding Properties

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