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Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
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Neuroscience
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JoVE Journal Neuroscience
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

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10,836 Views

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09:51 min

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July 15, 2012

DOI:

10.3791/3907-v

DOI:
10.3791/3907-v

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09:51 min
July 15, 2012

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2 Views
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1Fishberg Department of Neuroscience and Friedman Brain Institute,Mt. Sinai School of Medicine, 2Phase Five Communications Inc.

Summary

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We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

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Intracellular CalciumSynaptic EfficacyMollusc AplysiaCalcium ImagingIntracellular RecordingBuccal GanglionSynaptic PlasticityHomosynaptic FacilitationCalcium OrangeIontophoresisFluorescent Dye

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Cite this Article

Ludwar, B. C., Evans, C. G., Cropper, E. C. Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia. J. Vis. Exp. (65), e3907, doi:10.3791/3907 (2012).

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