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用于寡核苷酸传递的可生多孔硅纳米粒子的合成、功能化和表征
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JoVE Journal Engineering
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
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用于寡核苷酸传递的可生多孔硅纳米粒子的合成、功能化和表征

DOI:
10.3791/59440-v

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08:53 min

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April 16, 2019

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,
1Materials Science and Engineering Program,University of California, San Diego, 2Department of Chemistry and Biochemistry,University of California, San Diego

Chapters

  • 00:04Title
  • 00:42Synthesis of Porous Silicon Nanoparticles (pSiNPs)
  • 03:12Preparation of Fusogenic Lipid Film and Loading of siRNA in pSiNPs
  • 04:20Coating siRNA-loaded pSiNPs with Fusogenic Lipids
  • 05:42Conjugation of Targeting Peptides
  • 06:45Results: Characterization of Fusogenic Silicon Nanoparticles for Oligonucleotide Delivery
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

我们证明了富里多孔硅纳米粒子的合成, 以提高体外和体内寡核苷酸的传递效果。多孔硅纳米颗粒加入 siRNA 形成核心, 通过挤压包覆的杂原脂质形成外壳。包括定位功能化和粒子表征。

Tags

Porous Silicon NanoparticlesFusogenic NanoparticlesSiRNA DeliveryGene SilencingIn Vivo Gene ModulationHydrofluoric Acid EtchingPorous Silicon SynthesisNanoparticle Characterization

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