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Patojenler, Commensals ve ana bilgisayar bağırsak epitel arasındaki etkileşimler çalışmaya insan Colonoid Monolayers
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
DOI:

07:20 min

April 09, 2019

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Chapters

  • 00:04Title
  • 01:22Isolation and Dissociation of Enteroids/Colonoids from 3D Culture
  • 02:39Plating the Enteroid/Colonoid Suspension
  • 04:10Fixation to Preserve and Immunostain Mucus
  • 05:16Results: Establishment and Evaluation of Human Enteroid/Colonoid Monolayers
  • 06:51Conclusion

Summary

Automatic Translation

Burada, bir protokol kültür insan enteroid veya ana bilgisayar microbiota epitel etkileşimleri hücresel ve biyokimyasal düzeyde çalışmaya sağlam bariyer fonksiyonu var colonoid monolayers mevcut.

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