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Immunology and Infection
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Monocapas de Colonoid humano para estudiar las interacciones entre patógenos, comensales y epitelio Intestinal Host
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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Monocapas de Colonoid humano para estudiar las interacciones entre patógenos, comensales y epitelio Intestinal Host

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8,609 Views

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07:20 min

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April 09, 2019

DOI:

10.3791/59357-v

DOI:
10.3791/59357-v

•

07:20 min
April 09, 2019

•

1 Views
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1Department of Internal Medicine,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

Summary

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Aquí, presentamos un protocolo cultura humana enteroide o monocapas de colonoid que tienen la función de la barrera intacta para estudiar las interacciones epiteliales microbiota de host a nivel celular y bioquímico.

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Cite this Article

In, J. G., Foulke-Abel, J., Clarke, E., Kovbasnjuk, O. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (146), e59357, doi:10.3791/59357 (2019).

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