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Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

8,602 Views

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07:20 min

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April 09, 2019

DOI:

10.3791/59357-v

DOI:
10.3791/59357-v

•

07:20 min
April 09, 2019

•

1 Views
, , ,
1内科学科,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

概要

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Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

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Cite this Article

In, J. G., Foulke-Abel, J., Clarke, E., Kovbasnjuk, O. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (146), e59357, doi:10.3791/59357 (2019).

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