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A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

English

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7,512 Views

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08:42 min

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April 10, 2019

DOI:

10.3791/59194-v

DOI:
10.3791/59194-v

•

08:42 min
April 10, 2019

•

4 Views
, , , ,
1Department of Pediatrics,Northwestern University, 2Division of Pediatric Surgery,Ann & Robert H. Lurie Children’s Hospital of Chicago

Summary

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Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

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Cite this Article

Ares, G. J., Buonpane, C., Yuan, C., Wood, D., Hunter, C. J. A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis. J. Vis. Exp. (146), e59194, doi:10.3791/59194 (2019).

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