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Immunologie et infection
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A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
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Immunologie et infection
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Journal JoVE Immunologie et infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

DOI:
10.3791/59194-v

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08:42 min

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April 10, 2019

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, , , ,
1Department of Pediatrics,Northwestern University, 2Division of Pediatric Surgery,Ann & Robert H. Lurie Children’s Hospital of Chicago

Chapitres

  • 00:04Titre
  • 01:25Crypt Isolation and Plating from Whole Tissue
  • 05:21Induction of Experimental NEC and Preparation for Paraffin Embedding
  • 07:22Results: Analysis of Enteroids
  • 08:21Conclusion

Summary

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Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

Tags

Human Epithelial EnteroidsNecrotizing EnterocolitisIntestinal Stem CellsOrganoidsCell IsolationInflammationIntestinal EpitheliumCo-administrationLipopolysaccharide

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