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Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

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14,809 Views

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10:05 min

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January 25, 2017

DOI:

10.3791/54826-v

DOI:
10.3791/54826-v

•

10:05 min
January 25, 2017

•

8 Views
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1The Lloyd Rigler Sleep Apnea Research Laboratory, Unit of Anatomy and Cell Biology, The Ruth and Bruce Rappaport Faculty of Medicine,Technion-Israel Insitute of Technology

Summary

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We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.

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Cite this Article

Lavie, L., Dyugovskaya, L., Polyakov, A., Rogovoy, O., Leder, E. Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro. J. Vis. Exp. (119), e54826, doi:10.3791/54826 (2017).

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