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準備や髄脊髄小脳スライス培養の免疫染色

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

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準備や髄脊髄小脳スライス培養の免疫染色

10,573 Views

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09:41 min

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March 20, 2019

DOI:

10.3791/59163-v

DOI:

10.3791/59163-v

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09:41 min

March 20, 2019

•

6 Views

Melina Thetiot¹, Rémi Ronzano¹, Marie-Stéphane Aigrot¹, Catherine Lubetzki¹, Anne Desmazières¹

¹Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Summary

マウス小脳・髄鞘形成と中枢神経系検査のメカニズムを調査するため適切な免疫組織化学的染色ミエリン鞘からスライス培養切片を準備する方法をご紹介します。

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