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Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
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March 20, 2019
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Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.
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Thetiot, M., Ronzano, R., Aigrot, M., Lubetzki, C., Desmazières, A. Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures. J. Vis. Exp. (145), e59163, doi:10.3791/59163 (2019).
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