/
/
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
10,564 Views
•
09:41 min
•
March 20, 2019
DOI:
Summary
Automatically generated
Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.
Read Article
Cite this Article
Thetiot, M., Ronzano, R., Aigrot, M., Lubetzki, C., Desmazières, A. Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures. J. Vis. Exp. (145), e59163, doi:10.3791/59163 (2019).
Copy