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Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
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JoVE Journal 神経科学
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

DOI:
10.3791/59163-v

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09:41 min

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March 20, 2019

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, , , ,
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

章

  • 00:04標題
  • 00:36Cerebellum Dissection and Sections Preparation
  • 03:58Slices Culture and Demyelination
  • 04:55Immunohistochemistry
  • 08:15Results: Myelin Immunostaining in Organotypic Cerebellar Slices
  • 09:03Conclusion

概要

自動翻訳

自動翻訳

Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.

タグ

Myelinating Organotypic Cerebellar Slice CulturesMyelinationRemyelinationCerebellumDissectionImmunostaining

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