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タンパク質の折り畳み、トランスポート、および放射性パルス追跡による生体細胞の劣化の解析
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Biochemistry
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JoVE Journal Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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タンパク質の折り畳み、トランスポート、および放射性パルス追跡による生体細胞の劣化の解析

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10,899 Views

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:
10.3791/58952-v

•

08:59 min
February 12, 2019

•

11 Views
, , , , ,
1Cellular Protein Chemistry,Utrecht University, 2Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Summary

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ここで折りたたみ、トランスポート、および生きているセルで続かれるべき蛋白質の分解の速度論的解析を可能にする一般的なパルス追跡法のためのプロトコルについて述べる。

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Cite this Article

McCaul, N., Yeoh, H. Y., van Zadelhoff, G., Lodder, N., Kleizen, B., Braakman, I. Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase. J. Vis. Exp. (144), e58952, doi:10.3791/58952 (2019).

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