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Analyse van de vouwing van eiwitten, vervoer en afbraak in levende cellen door radioactieve Pulse Chase
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Biochemistry
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JoVE Journal Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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Analyse van de vouwing van eiwitten, vervoer en afbraak in levende cellen door radioactieve Pulse Chase

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10,896 Views

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:
10.3791/58952-v

•

08:59 min
February 12, 2019

•

11 Views
, , , , ,
1Cellular Protein Chemistry,Utrecht University, 2Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Summary

Automatically generated

Hier beschrijven we een protocol voor een algemene pulse-chase-methode, waarmee de kinetische analyse van vouwing, vervoer en afbraak van eiwitten te volgen in levende cellen.

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Cite this Article

McCaul, N., Yeoh, H. Y., van Zadelhoff, G., Lodder, N., Kleizen, B., Braakman, I. Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase. J. Vis. Exp. (144), e58952, doi:10.3791/58952 (2019).

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