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Analyse der Proteinfaltung, Transport und Abbau in lebenden Zellen durch radioaktive Puls Chase
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Biochemistry
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JoVE Journal Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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Analyse der Proteinfaltung, Transport und Abbau in lebenden Zellen durch radioaktive Puls Chase

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10,899 Views

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:
10.3791/58952-v

•

08:59 min
February 12, 2019

•

11 Views
, , , , ,
1Cellular Protein Chemistry,Utrecht University, 2Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Summary

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Hier beschreiben wir ein Protokoll für eine allgemeine Puls-Jagd-Methode, die die kinetische Analyse der Faltung, Transport und Abbau von Proteinen in lebenden Zellen einzuhaltenden erlaubt.

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Cite this Article

McCaul, N., Yeoh, H. Y., van Zadelhoff, G., Lodder, N., Kleizen, B., Braakman, I. Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase. J. Vis. Exp. (144), e58952, doi:10.3791/58952 (2019).

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