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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

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Biochimie

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Journal JoVE Biochimie

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

10,812 Views

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08:59 min

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February 12, 2019

DOI:

10.3791/58952-v

DOI:

10.3791/58952-v

•

08:59 min

February 12, 2019

•

2 Views

Nicholas McCaul², Hui Ying Yeoh, Guus van Zadelhoff, Naomi Lodder, Bertrand Kleizen, Ineke Braakman

¹Cellular Protein Chemistry,Utrecht University, ²Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Tags

Protein Folding Protein Transport Protein Degradation Radioactive Pulse Chase 35S-labeled Methionine And Cysteine Protein Biosynthesis Co- And Post-translational Modifications Disulfide Bond Formation Acetylation Cell Culture Transfection Starvation Medium Pulse Solution Chase Medium Stop Buffer

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