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Fastsettelse av regulatoriske T-celle delsett i Murine Thymus, bukspyttkjertelen drenering lymfeknute og milt bruker flowcytometri
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
DOI:

08:06 min

February 27, 2019

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Chapters

  • 00:04Title
  • 00:29Harvesting Organs from Animals
  • 01:05Single Cell Isolation from Thymus and Spleen
  • 02:41Single Cell Isolation from PDLN
  • 03:58Flow Cytometry
  • 06:07Results: Determination of the Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node, and Spleen
  • 07:36Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for å forberede enkeltceller fra murine thymus, bukspyttkjertelen drenering lymfeknute og milt å ytterligere studere disse celler ved hjelp av flowcytometri. I tillegg ble denne protokollen brukt for å bestemme delsett av regulatoriske T celler ved hjelp av flowcytometri.

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