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Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
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Chapters
- 00:04Title
- 00:29Harvesting Organs from Animals
- 01:05Single Cell Isolation from Thymus and Spleen
- 02:41Single Cell Isolation from PDLN
- 03:58Flow Cytometry
- 06:07Results: Determination of the Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node, and Spleen
- 07:36Conclusion
Herein, we present a protocol to prepare single cells from murine thymus, pancreatic draining lymph node and spleen to further study these cells using flow cytometry. In addition, this protocol was used for determining the subsets of regulatory T cells using flow cytometry.
