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Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
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JoVE 杂志 免疫与感染
Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

DOI:
10.3791/58848-v

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08:06 min

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February 27, 2019

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, , ,
1Department of Medical Cell Biology,Uppsala University

Chapters

  • 00:04Title
  • 00:29Harvesting Organs from Animals
  • 01:05Single Cell Isolation from Thymus and Spleen
  • 02:41Single Cell Isolation from PDLN
  • 03:58Flow Cytometry
  • 06:07Results: Determination of the Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node, and Spleen
  • 07:36Conclusion

Summary

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Herein, we present a protocol to prepare single cells from murine thymus, pancreatic draining lymph node and spleen to further study these cells using flow cytometry. In addition, this protocol was used for determining the subsets of regulatory T cells using flow cytometry.

Tags

Flow CytometryRegulatory T CellsThymusPancreatic Draining Lymph NodeSpleenImmune CellsCell IsolationCell SuspensionCell LysisCell Quantification

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