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Purificação de H3 e H4 histona proteínas e a quantificação do Histone acetilado marcas em células e tecidos cerebrais
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Purificação de H3 e H4 histona proteínas e a quantificação do Histone acetilado marcas em células e tecidos cerebrais

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20,611 Views

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09:43 min

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November 30, 2018

DOI:

10.3791/58648-v

DOI:
10.3791/58648-v

•

09:43 min
November 30, 2018

•

8 Views
, ,
1Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation,University of Miami Miller School of Medicine

Summary

Automatically generated

O objetivo deste artigo é fornecer um guia abrangente, sistemático para a purificação eficiente das histonas H3 e H4 e quantificação dos resíduos de histona acetilado.

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Cite this Article

Janczura, K. J., Volmar, C., Wahlestedt, C. Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue. J. Vis. Exp. (141), e58648, doi:10.3791/58648 (2018).

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