Rivista
/
Neuroscienze
/
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

20,675 Views

•

09:43 min

•

November 30, 2018

DOI:

10.3791/58648-v

DOI:
10.3791/58648-v

•

09:43 min
November 30, 2018

•

8 Views
, ,
1Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation,University of Miami Miller School of Medicine

Summary

Automatically generated

The purpose of this article is to provide a comprehensive, systematic guide to the efficient purification of histones H3 and H4 and the quantification of acetylated histone residues.

Explore More Videos

Histone ProteinsH3H4AcetylationPost-translational ModificationsPurificationQuantificationCell ExtractsAlzheimer’s DiseaseEpigenetic RegulationCell CultureCentrifugationBuffer ExtractionPH Neutralization

Read Article

Cite this Article

Janczura, K. J., Volmar, C., Wahlestedt, C. Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue. J. Vis. Exp. (141), e58648, doi:10.3791/58648 (2018).

Download .ris file

Copy

Share Video

.

Copy