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Zuivering van H3 en H4 Histone eiwitten en de kwantificering van geacetyleerd Histone merken in cellen en hersenweefsel
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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Zuivering van H3 en H4 Histone eiwitten en de kwantificering van geacetyleerd Histone merken in cellen en hersenweefsel

Nederlands

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20,611 Views

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09:43 min

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November 30, 2018

DOI:

10.3791/58648-v

DOI:
10.3791/58648-v

•

09:43 min
November 30, 2018

•

8 Views
, ,
1Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation,University of Miami Miller School of Medicine

Summary

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Het doel van dit artikel is om een uitgebreide, systematische handleiding voor de efficiënte reiniging van histones H3 en H4 en de kwantificering van residuen geacetyleerd Histon.

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Cite this Article

Janczura, K. J., Volmar, C., Wahlestedt, C. Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue. J. Vis. Exp. (141), e58648, doi:10.3791/58648 (2018).

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