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H3 ・ H4 ヒストン蛋白質細胞および脳組織におけるアセチル化ヒストンの定量化の浄化
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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H3 ・ H4 ヒストン蛋白質細胞および脳組織におけるアセチル化ヒストンの定量化の浄化

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20,611 Views

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09:43 min

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November 30, 2018

DOI:

10.3791/58648-v

DOI:
10.3791/58648-v

•

09:43 min
November 30, 2018

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8 Views
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1Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation,University of Miami Miller School of Medicine

Summary

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この資料の目的は、ヒストン H3、H4 の効率的な精製と残基のアセチル化ヒストンの定量化に包括的、体系的なガイドを提供することです。

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Cite this Article

Janczura, K. J., Volmar, C., Wahlestedt, C. Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue. J. Vis. Exp. (141), e58648, doi:10.3791/58648 (2018).

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