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Developmental Biology
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成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
Please note that all translations are automatically generated.
Click here for the English version.
成人小鼠成纤维细胞对红细胞生成子细胞的直接线重编程
DOI:
10.3791/58464-v
•
11:46 min
•
December 14, 2018
•
Melissa Ilsley
,
Sandra Capellera-Garcia
,
Kishori Dhulipala
,
Alban Johansson
,
Johan Flygare
1
Department of Molecular Medicine and Gene Therapy
,
Lund University
Chapters
00:04
Title
01:25
Establishment and Maintenance of Primary Mouse Tail Tip Fibroblast Cultures
05:13
Retrovirus Production
07:10
GTLM Transduction and iEP Harvest
08:52
Results: Reprogramming of Induced Erythroid Progenitors (iEPs)
11:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了我们的协议, 产生诱导红血球祖细胞 (iep) 从小鼠成纤维细胞使用转录因子驱动的直接谱系重新编程 (dlr)。
Tags
Direct Lineage Reprogramming
Fibroblasts
Erythroid Progenitor Cells
Transcriptional Regulation
In Vitro
Tail-tip Fibroblasts
Transfusion Medicine
Chromatin Modifications
Gene Expression Changes
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