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Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

DOI:
10.3791/58464-v

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11:46 min

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December 14, 2018

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, , , ,
1Department of Molecular Medicine and Gene Therapy,Lund University

Capitoli

  • 00:04Titolo
  • 01:25Establishment and Maintenance of Primary Mouse Tail Tip Fibroblast Cultures
  • 05:13Retrovirus Production
  • 07:10GTLM Transduction and iEP Harvest
  • 08:52Results: Reprogramming of Induced Erythroid Progenitors (iEPs)
  • 11:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present our protocol for producing induced erythroid progenitors (iEPs) from mouse adult fibroblasts using transcription factor-driven direct lineage reprogramming (DLR).

Tags

Direct Lineage ReprogrammingFibroblastsErythroid Progenitor CellsTranscriptional RegulationIn VitroTail-tip FibroblastsTransfusion MedicineChromatin ModificationsGene Expression Changes

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