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Inhibición de la proteína inducible y Reversible dominante negativo (DN)
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Genetics
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JoVE Journal Genetics
Inducible and Reversible Dominant-negative (DN) Protein Inhibition
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Inhibición de la proteína inducible y Reversible dominante negativo (DN)

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8,219 Views

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08:35 min

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January 07, 2019

DOI:

10.3791/58419-v

DOI:
10.3791/58419-v

•

08:35 min
January 07, 2019

•

7 Views
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1Department of Oral Biology,Creighton University School of Dentistry

Summary

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Aquí presentamos un protocolo para desarrollar un sistema inducible negativo dominante, en el que cualquier proteína puede ser condicional inactivada por overexpressing reversiblemente una versión mutante dominante negativo de él.

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Cite this Article

Tarang, S., Pyakurel, U., Doi, S. M., Weston, M. D., Rocha-Sanchez, S. M. Inducible and Reversible Dominant-negative (DN) Protein Inhibition. J. Vis. Exp. (143), e58419, doi:10.3791/58419 (2019).

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