Here we present a protocol to develop a dominant-negative inducible system, in which any protein can be conditionally inactivated by reversibly overexpressing a dominant-negative mutant version of it.
Tarang, S., Pyakurel, U., Doi, S. M., Weston, M. D., Rocha-Sanchez, S. M. Inducible and Reversible Dominant-negative (DN) Protein Inhibition. J. Vis. Exp. (143), e58419, doi:10.3791/58419 (2019).