Journal
/
Genética
/
Inducible and Reversible Dominant-negative (DN) Protein Inhibition
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

DOI:
10.3791/58419-v

•

08:35 min

•

January 07, 2019

•

, , , ,
1Department of Oral Biology,Creighton University School of Dentistry

Capítulos

  • 00:04Título
  • 00:58In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in NIH3T3 Cells
  • 02:19In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in HEI-OC1 Cells
  • 05:37Generation of CBRb+/ROSA-CAG-rtTA+ (CBRb) Transgenic Mice and In Vivo Testing of the DN-CBRb Approach
  • 06:09Results: Confirmation of Efficiency of TetO-DN-CB-myc6-Rb1 Construct
  • 07:50Conclusion

Summary

Tadução automática

Tadução automática

Here we present a protocol to develop a dominant-negative inducible system, in which any protein can be conditionally inactivated by reversibly overexpressing a dominant-negative mutant version of it.

Tags

Dominant-negative ProteinConditional InactivationTransient InactivationPartial AblationEndogenous ExpressionMonomeric ProteinsNIH3T3 CellsPTet-SplicePCMV-Tet3GLipid-based TransfectionHEI-OC1 CellsCell ProliferationDoxycycline Induction

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Generation of Fluorescent Protein Fusions in Candida Species

Controllable Ion Channel Expression through Inducible Transient Transfection

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

An Assay for Quantifying Protein-RNA Binding in Bacteria

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Read Article