Dergi
/
Biyokimya
/
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

10,057 Views

•

12:25 min

•

September 28, 2018

DOI:

10.3791/58393-v

DOI:
10.3791/58393-v

•

12:25 min
September 28, 2018

•

1 Views
, , ,
1Department of Plant Biology,University of California – Davis

Özet

Automatically generated

We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

Explore More Videos

ThylakoidsProtein TransportChloroplast MembranesProtein TargetingPercoll GradientChloroplast IsolationPea ShootsHomogenizationCentrifugationChlorophyll Concentration

Read Article

Cite this Article

Asher, A., Ganesan, I., Klasek, L., Theg, S. M. Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays. J. Vis. Exp. (139), e58393, doi:10.3791/58393 (2018).

Download .ris file

Copy

Share Video

.

Copy