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Journal
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Biochemistry
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Biosynthesized l-dihydroxyphenylalanine (多巴) 的遗传合并及其在蛋白质共轭中的应用
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Biochemistry
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JoVE Journal
Biochemistry
Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation
Please note that all translations are automatically generated.
Click here for the English version.
Biosynthesized l-dihydroxyphenylalanine (多巴) 的遗传合并及其在蛋白质共轭中的应用
DOI:
10.3791/58383-v
•
10:24 min
•
August 24, 2018
•
Sanggil Kim
,
Hyun Soo Lee
1
Department of Chemistry
,
Sogang University
Chapters
00:04
Title
00:29
Culture Preparation
02:06
Expression and Purification of GFP-E90DOPA by a Biosynthetic System
04:25
Oligomerization of Purified GFP-E90DOPA and SDS-PAGE Analysis
05:47
Conjugation of GFP-E90DOPA with an Alkyne Probe by SPOCQ, Purification of the Labeled GFP
07:10
SDS-PAGE Analysis and Fluorescence Gel Scanning of Labeled GFP
08:21
Results: Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine
10:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
在此, 我们提出了一种从简单的起始材料中 dihydroxyphenylalanine biosynthesized 的遗传方法及其在蛋白质共轭中的应用的协议。
Tags
Genetic Incorporation
L-dihydroxyphenylalanine (DOPA)
Protein Conjugation
Biosynthetic System
E. Coli
Expression Plasmid
Electroporation
LB Medium
PA-5052 Medium
Cell Lysis
Protein Purification
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