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Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation
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生物化学
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Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation

7,670 Views

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10:24 min

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August 24, 2018

DOI:

10.3791/58383-v

DOI:
10.3791/58383-v

•

10:24 min
August 24, 2018

•

4 Views
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1Department of Chemistry,Sogang University

Tags

Genetic IncorporationL-dihydroxyphenylalanine (DOPA)Protein ConjugationBiosynthetic SystemE. ColiExpression PlasmidElectroporationLB MediumPA-5052 MediumCell LysisProtein Purification

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Kim, S., Lee, H. S. Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation. J. Vis. Exp. (138), e58383, doi:10.3791/58383 (2018).

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