Journal
/
生物化学
/
Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation

7,707 Views

•

10:24 min

•

August 24, 2018

DOI:

10.3791/58383-v

DOI:
10.3791/58383-v

•

10:24 min
August 24, 2018

•

10 Views
,
1Department of Chemistry,Sogang University

Summary

Automatically generated

Here, we present a protocol for the genetic incorporation of L-dihydroxyphenylalanine biosynthesized from simple starting materials and its application to protein conjugation.

Explore More Videos

Genetic IncorporationL-dihydroxyphenylalanine (DOPA)Protein ConjugationBiosynthetic SystemE. ColiExpression PlasmidElectroporationLB MediumPA-5052 MediumCell LysisProtein Purification

Read Article

Cite this Article

Kim, S., Lee, H. S. Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation. J. Vis. Exp. (138), e58383, doi:10.3791/58383 (2018).

Download .ris file

Copy

Share Video

.

Copy