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A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
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Biochemistry
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JoVE Journal Biochemistry
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

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8,193 Views

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05:43 min

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January 24, 2017

DOI:

10.3791/55077-v

DOI:
10.3791/55077-v

•

05:43 min
January 24, 2017

•

3 Views
, , , ,
1Graduate School of Frontier Biosciences,Osaka University, 2Department of Biotechnology and Genetic Engineering,Jahangirnagar University, 3Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences,Doshisha University

Summary

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We have established a method for the purification of coregulatory interaction proteins using the LC-MS/MS system.

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Cite this Article

Tsuchiya, M., Karim, M. R., Matsumoto, T., Ogawa, H., Taniguchi, H. A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis. J. Vis. Exp. (119), e55077, doi:10.3791/55077 (2017).

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