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C. elegans Germline timidin Analog EdU ile hücre döngüsü analizi
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Developmental Biology
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JoVE Journal Developmental Biology
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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C. elegans Germline timidin Analog EdU ile hücre döngüsü analizi

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11,098 Views

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06:58 min

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October 22, 2018

DOI:

10.3791/58339-v

DOI:
10.3791/58339-v

•

06:58 min
October 22, 2018

•

3 Views
, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Summary

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Bir görüntüleme tabanlı yöntemi anlatılan S fazlı tanımlamak ve timidin kullanarak C. elegans hermafrodit germline hücre döngüsü dinamiklerini analiz etmek için kullanılabilir analog EdU. Bu yöntem yok transgenes gerektirir ve immünfloresan boyama ile uyumludur.

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Cite this Article

Kocsisova, Z., Mohammad, A., Kornfeld, K., Schedl, T. Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU. J. Vis. Exp. (140), e58339, doi:10.3791/58339 (2018).

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