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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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JoVE Journal 発生生物学
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

DOI:
10.3791/58339-v

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06:58 min

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October 22, 2018

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, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2遺伝学科,Washington University School of Medicine, St. Louis, Missouri

章

  • 00:04標題
  • 00:505-ethynyl-2′-deoxyuridine (EdU)-labeled Bacteria Preparation and C. elegans Feeding
  • 02:46Germline Dissection, Fixation, Rehydration, and EdU Click Reaction/Detection
  • 05:09Results: Representative C. elegans Cell Cycle Analyses
  • 06:20Conclusion

概要

自動翻訳

自動翻訳

An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

タグ

Cell Cycle AnalysisC. ElegansGermlineEdUThymidine AnalogImmunofluorescent StainingGerm Line Stem CellCell Cycle DynamicsAgingNutritionAltered GenotypesIdiodisprepationHematoxylin StainingSterile TechniqueM9 BufferE. ColiEdU SupplementationCentrifugationNematode Growth MediumPBSDissectionFixation

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