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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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JoVE Journal Biologia dello sviluppo
Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

11,098 Views

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06:58 min

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October 22, 2018

DOI:

10.3791/58339-v

DOI:
10.3791/58339-v

•

06:58 min
October 22, 2018

•

3 Views
, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Summary

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An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

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Kocsisova, Z., Mohammad, A., Kornfeld, K., Schedl, T. Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU. J. Vis. Exp. (140), e58339, doi:10.3791/58339 (2018).

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