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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU
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发育生物学
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Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

11,085 Views

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06:58 min

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October 22, 2018

DOI:

10.3791/58339-v

DOI:
10.3791/58339-v

•

06:58 min
October 22, 2018

•

3 Views
, , ,
1Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, 2Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Summary

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An imaging-based method is described that can be used to identify S-phase and analyze cell cycle dynamics in the C. elegans hermaphrodite germline using the thymidine analog EdU. This method requires no transgenes and is compatible with immunofluorescent staining.

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Kocsisova, Z., Mohammad, A., Kornfeld, K., Schedl, T. Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU. J. Vis. Exp. (140), e58339, doi:10.3791/58339 (2018).

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