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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白

DOI:

10.3791/58283-v

•

09:14 min

•

August 20, 2018

•

Sayan Chakraborty, Krittin Trihemasava, Guozhou Xu

¹Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Chapters

00:04Title
00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
07:57Conclusion

Summary

Automatic Translation

在这里, 我们提出了利用昆虫细胞和杆状病毒蛋白表达系统来产生大量植物分泌蛋白的蛋白质结晶的协议。用 GP67 或昆虫 hemolin 信号肽对昆虫细胞中植物蛋白分泌表达进行了改进, 并对杆状病毒表达载体进行了修正。

Tags

Baculovirus Expression Vectors Secreted Plant Proteins Insect Cells Protein Structure Determination Recombinant Proteins DNA Cloning DNA Sequencing Bacterial Transformation Bacmid DNA Sf9 Cells

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