00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.