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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化

DOI:
10.3791/58279-v

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10:07 min

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October 24, 2018

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, , , ,
1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Chapters

  • 00:04Title
  • 00:41Step I: Corneal Induction in Suspension Culture
  • 03:07Step II: Corneal Differentiation in Adherent Culture
  • 04:33Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 06:08Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 07:25Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
  • 09:31Conclusion

Summary

Automatic Translation

Automatic Translation

本协议介绍了一种简单的两步法在异种和无细胞培养条件下, 从人多潜能干细胞中分化角膜缘上皮干细胞的方法。这里介绍的细胞培养方法可实现经济高效的大规模生产, 适用于角膜细胞治疗使用的临床质量细胞。

Tags

Corneal Limbal Epithelial Stem CellsHuman Pluripotent Stem CellsFeeder-freeEmbryoid Body FormationBasal Induction MediumSB-505124Human Basic Fibroblast Growth FactorLimbal Stem Cell DeficiencyCorneal Cell Replacement Therapy

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