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Developmental Biology

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JoVE Journal Developmental Biology
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
 

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Article DOI: 10.3791/58279-v • 10:07 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 1
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere
* These authors contributed equally

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요약
Automatic Translation

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

Tags

Corneal Limbal Epithelial Stem Cells Human Pluripotent Stem Cells Feeder Cell Free Method Corneal Stem Cell Deficiency Corneal Cell Replacement Therapies Embryoid Body Formation Xeno Free Trypsin EDTA
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