Rivista
/
Biologia dello sviluppo
/
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

DOI:
10.3791/58279-v

•

10:07 min

•

October 24, 2018

•

, , , ,
1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Capitoli

  • 00:04Titolo
  • 00:41Step I: Corneal Induction in Suspension Culture
  • 03:07Step II: Corneal Differentiation in Adherent Culture
  • 04:33Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 06:08Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 07:25Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
  • 09:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

Tags

Corneal Limbal Epithelial Stem CellsHuman Pluripotent Stem CellsFeeder-freeEmbryoid Body FormationBasal Induction MediumSB-505124Human Basic Fibroblast Growth FactorLimbal Stem Cell DeficiencyCorneal Cell Replacement Therapy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation

Read Article