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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

7,463 Views

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10:07 min

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October 24, 2018

DOI:

10.3791/58279-v

DOI:
10.3791/58279-v

•

10:07 min
October 24, 2018

•

1 Views
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1BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Summary

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This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

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Cite this Article

Hongisto, H., Vattulainen, M., Ilmarinen, T., Mikhailova, A., Skottman, H. Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (140), e58279, doi:10.3791/58279 (2018).

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