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Arbovirus infecties als Screening van hulpmiddelen voor de identificatie van de virale Immunomodulators en Host antivirale factoren
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Immunology and Infection
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JoVE Journal Immunology and Infection
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
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Arbovirus infecties als Screening van hulpmiddelen voor de identificatie van de virale Immunomodulators en Host antivirale factoren

Nederlands

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6,698 Views

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06:02 min

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September 13, 2018

DOI:

10.3791/58244-v

DOI:
10.3791/58244-v

•

06:02 min
September 13, 2018

•

1 Views
, ,
1Department of Microbiology,University of Texas Southwestern Medical Center

Summary

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Hier presenteren we de protocollen om te identificeren 1) virus-gecodeerde immunomodulators ter bevordering van arbovirus replicatie en 2) eukaryotische gastheer factoren die arbovirus replicatie beperken. Deze fluorescentie - en luminescentie gebaseerde methoden toestaan onderzoekers snel verkrijgen van kwantitatieve uitlezingen van arbovirus replicatie in simplistische testen met lage signaal-ruis verhouding.

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Rex, E. A., Seo, D., Gammon, D. B. Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors. J. Vis. Exp. (139), e58244, doi:10.3791/58244 (2018).

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