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La ricostituzione delle oscillazioni del ciclo cellulare in microemulsioni di uovo senza cellula Xenopus estrae
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Biochemistry
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JoVE Journal Biochemistry
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
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La ricostituzione delle oscillazioni del ciclo cellulare in microemulsioni di uovo senza cellula Xenopus estrae

italiano

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8,025 Views

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06:31 min

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September 27, 2018

DOI:

10.3791/58240-v

DOI:
10.3791/58240-v

•

06:31 min
September 27, 2018

•

7 Views
, , , ,
1Department of Biophysics,University of Michigan, Ann Arbor, 2Department of Chemistry,University of Michigan, Ann Arbor, 3Department of Computer Science,Wayne State University, 4Department of Physics,University of Michigan, Ann Arbor

Summary

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Presentiamo un metodo per la generazione di in vitro autosostenuto mitotica oscillazioni a livello di singola cellula incapsulando gli estratti di uovo di Xenopus laevis in microemulsioni di acqua in olio.

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Cell-cycle OscillationsXenopus Egg ExtractsMicroemulsionsCell-free SystemMitotic NetworkCell Cycle ClockHigh-throughputQuantitative ManipulationCell ActivationCell LysisCell Extract Preparation

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Cite this Article

Guan, Y., Wang, S., Jin, M., Xu, H., Yang, Q. Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts. J. Vis. Exp. (139), e58240, doi:10.3791/58240 (2018).

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