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Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
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生物化学
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JoVE 杂志 生物化学
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

DOI:
10.3791/58240-v

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06:31 min

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September 27, 2018

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, , , ,
1Department of Biophysics,University of Michigan, Ann Arbor, 2Department of Chemistry,University of Michigan, Ann Arbor, 3Department of Computer Science,Wayne State University, 4Department of Physics,University of Michigan, Ann Arbor

Chapters

  • 00:04Title
  • 00:41Preparation of Cycling Xenopus Extracts
  • 03:39Droplet Generation and Imaging
  • 05:19Results: Cell-cycle System Capable of Reconstructing Mitotic Oscillator of Cdk1 and APC/C
  • 05:53Conclusion

Summary

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We present a method for the generation of in vitro self-sustained mitotic oscillations at the single-cell level by encapsulating egg extracts of Xenopus laevis in water-in-oil microemulsions.

Tags

Keywords: Cell-cycle OscillationsXenopus Egg ExtractsMicroemulsionsCell-free SystemMitotic NetworkCell Cycle ClockHigh-throughputQuantitative ManipulationCell ActivationCell LysisCell Extract Preparation

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