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Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
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Bioquímica
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JoVE Journal Bioquímica
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

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06:31 min

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September 27, 2018

DOI:

10.3791/58240-v

DOI:
10.3791/58240-v

•

06:31 min
September 27, 2018

•

7 Views
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1Department of Biophysics,University of Michigan, Ann Arbor, 2Department of Chemistry,University of Michigan, Ann Arbor, 3Department of Computer Science,Wayne State University, 4Department of Physics,University of Michigan, Ann Arbor

Summary

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We present a method for the generation of in vitro self-sustained mitotic oscillations at the single-cell level by encapsulating egg extracts of Xenopus laevis in water-in-oil microemulsions.

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Cell-cycle OscillationsXenopus Egg ExtractsMicroemulsionsCell-free SystemMitotic NetworkCell Cycle ClockHigh-throughputQuantitative ManipulationCell ActivationCell LysisCell Extract Preparation

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Guan, Y., Wang, S., Jin, M., Xu, H., Yang, Q. Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts. J. Vis. Exp. (139), e58240, doi:10.3791/58240 (2018).

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